#Cyclic amp assay manual

Intra-assay precision for duplicate samples is typically 5% or less. This is especially helpful in cell-based assays when measuring Gs- or Gi-coupled agonist or antagonist stimulation and/or inhibition. The assay has a wide dynamic range, detecting from 0.06 to 6,000 picomoles of cAMP without the need for sample dilution or manipulations such as acetylation. As few as 60 femtomoles of cAMP can be detected. This chemiluminescent assay is designed to provide the highest sensitivity of any commercially available cAMP assay. High Sensitivity and Hours of Steady Glow Time Useful for a wide range of receptor activation studies.Hours of read time with little or no degradation of the signal.The highest sensitivity of any commercially available cAMP assay.This assay is mainly used in secondary screening and pre-clinical research, where sensitivity and no false positives are essential. The chemiluminescent substrate/enhancer formulation is a ready-to-use reagent that generates a sustained-glow light emission from 30 minutes after addition. The cAMP assay utilizes the highly sensitive chemiluminescent CSPD™ Substrate with Sapphire-II™ Enhancer that is triggered by an enzyme conjugate composed of cAMP bound to alkaline phosphatase (cAMP-AP).

#Cyclic amp assay manual

This competitive chemiluminescent immunoassay is formatted with maximum flexibility to permit either manual reagent additions or automated high-throughput processing. The cAMP-Screen™ 96-well Cyclic AMP Immunoassay System enables ultrasensitive determination of cyclic AMP (cAMP) levels in cell lysates.